Syncytiotrophoblastic localization of the human growth hormone variant mRNA in the placenta.

Abstract

The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental growth hormone, which replaces pituitary GH in maternal blood throughout pregnancy. By means of mRNA competitive hybridization using 32P-labelled and unlabelled 30 bases long oligonucleotides, we first optimized specific hybridization conditions. In situ hybridization was then performed to locate the GH-V mRNA encoding placental growth hormone. The hGH-V gene appears expressed in the placental syncytiotrophoblast. Unlike the CS-A and CS-B genes (both encoding hPL) which are expressed uniformly in the syncytiotrophoblast, the GH-V mRNA is located in a few syncytiotrophoblast cells only.

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@article{Scippo1993SyncytiotrophoblasticLO, title={Syncytiotrophoblastic localization of the human growth hormone variant mRNA in the placenta.}, author={M L Scippo and Françis Frankenne and Elisabeth L. Hooghe-Peters and Ahmed Igout and Brigitte Velkeniers and Georges P Hennen}, journal={Molecular and cellular endocrinology}, year={1993}, volume={92 2}, pages={R7-13} }