Synchronization of porcine oocyte meiosis using cycloheximide and its application to the study of regulation by cumulus cells.

@article{Ye2002SynchronizationOP,
  title={Synchronization of porcine oocyte meiosis using cycloheximide and its application to the study of regulation by cumulus cells.},
  author={Jin Bo Ye and Anthony P F Flint and Keith H. S. Campbell and Martin Richard Luck},
  journal={Reproduction, fertility, and development},
  year={2002},
  volume={14 7-8},
  pages={433-42}
}
This paper describes the use of the protein synthesis inhibitor cycloheximide (CHX) to synchronize nuclear progression during meiotic maturation in porcine oocytes, and also the time-dependence of nuclear maturation on exposure of the oocyte to cumulus cells. Prior to culture, the majority of oocytes were at the germinal vesicle (GV) stage (95-100%), but distributed from GVI to GVIV (GVI 56.1 +/- 9.1%, GVII 15.3 +/- 1.4%, GVIII 21.5 +/- 7.1%, GVIV 7.1 +/- 3.5%). During culture of cumulus… CONTINUE READING