Synchronization of GABAergic interneuronal network in CA3 subfield of neonatal rat hippocampal slices.

@article{Khazipov1997SynchronizationOG,
  title={Synchronization of GABAergic interneuronal network in CA3 subfield of neonatal rat hippocampal slices.},
  author={Roustem Khazipov and Xavier Leinekugel and Ilgam Khalilov and Jean-Luc Gaiarsa and Yezekiel Ben‐Ari},
  journal={The Journal of Physiology},
  year={1997},
  volume={498}
}
1. Cell‐attached and whole‐cell recordings from interneurons localized in the stratum radiatum of the CA3 subfield (SR‐CA3) of neonatal (postnatal days 2‐5) rat hippocampal slices were performed to study their activity during the generation of GABAergic giant depolarizing potentials (GDPs) in CA3 pyramidal cells. 2. Dual recordings revealed that during the generation of GDPs in CA3 pyramidal cells, the interneurons fire bursts of spikes, on average 4.5 +/‐ 1.4 spikes per burst (cell‐attached… Expand
Origin of the synchronized network activity in the rabbit developing hippocampus
TLDR
By recording from isolated areas it is found that CA1, CA3 and the fascia dentata can produce GDPs, suggesting that they emerge as a property of local circuits present throughout the hippocampus. Expand
Developmental profile and synaptic origin of early network oscillations in the CA1 region of rat neonatal hippocampus
TLDR
The results demonstrate the presence of periodic and spontaneous Ca2+ transients in the majority of pyramidal cells and interneurones of the neonatal CA1 hippocampal network. Expand
Spontaneous recurrent network activity in organotypic rat hippocampal slices
Organotypic hippocampal slices were prepared from postnatal day 4 rats and maintained in culture for >6 weeks. Cultured slices exhibited from 12 days in vitro spontaneous events which closelyExpand
Heterogeneous populations of cells mediate spontaneous synchronous bursting in the developing hippocampus through a frequency-dependent mechanism
TLDR
The results strongly suggest that giant depolarizing potentials are locally generated from different initiation sites and not from a single region, in view of the principles underlying hyperexcitability in hippocampal slices, i.e. the intrinsic firing properties of individual cells and the connectivity patterns. Expand
GABAergic control of CA3-driven network events in the developing hippocampus.
TLDR
In the neonatal rat hippocampus, spontaneously occurring network events known as "Giant Depolarizing Potentials" (GDPs) are seen in vitro at a stage when GABAergic transmission is depolarizing, and appear to be an immature form of hippocampal sharp waves. Expand
Depolarizing GABA Acts on Intrinsically Bursting Pyramidal Neurons to Drive Giant Depolarizing Potentials in the Immature Hippocampus
TLDR
It is demonstrated here that CA3 pyramidal neurons in the newborn rat generate intrinsic bursts when depolarized, and the characteristic rhythmicity of GDP generation is not based on a temporally patterned output of the GABAergic interneuronal network. Expand
Giant Depolarizing Potentials: the Septal Pole of the Hippocampus Paces the Activity of the Developing Intact Septohippocampal ComplexIn Vitro
TLDR
Combined whole-cell and extracellular field recordings indicated that spontaneous GDPs are most often initiated in the septal poles of hippocampus and propagate to medial septum and temporal poles of both hippocampi simultaneously, suggesting hippocampal origin of GDPs. Expand
Use-dependent shift from inhibitory to excitatory GABAA receptor action in SP-O interneurons in the rat hippocampal CA3 area.
TLDR
Results here implicate the rapid activity-dependent degradation of hyperpolarizing IPSPs in SP-O interneurons in setting the temporal limits for a given interneuron to participate in beta-gamma oscillations synchronized by GABAergic synapses, implying that mutual GABAergic excitation provided by interneURons may be an integral part in the function of neuronal networks. Expand
Synchronized network activity in developing rat hippocampus involves regional hyperpolarization‐activated cyclic nucleotide‐gated (HCN) channel function
TLDR
An HCN1 spatiotemporal expression pattern is demonstrated in both CA3 principal cells and interneurons that correlates with the developmental profile of GDPs, suggesting a pivotal role for HCN channels expressed by CA3 neurons, and particularly CA3 pyramidal cells, in GDP‐related network synchronization. Expand
Giant Depolarizing Potentials Trigger Transient Changes in the Intracellular Cl- Concentration in CA3 Pyramidal Neurons of the Immature Mouse Hippocampus
TLDR
It is concluded that Cl--fluxes via GABAA receptors during GDPs induced substantial [Cl-]i changes and that this activity-dependent ionic plasticity in neuronal [Cl -]i contributes to the functional consequences of GABAergic responses, emphasizing the concept that [Cl--i is a state- and compartment-dependent parameter of individual cells. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 39 REFERENCES
Giant synaptic potentials in immature rat CA3 hippocampal neurones.
TLDR
In neurones in which evoked GDPs were blocked by bicuculline, a NMDA‐mediated component was revealed by increasing the strength or the frequency of stimulation, and during the second week of postnatal life, superfusion with bicuciulline induced, as in adult slices, interictal discharges. Expand
Synaptic GABAA activation induces Ca2+ rise in pyramidal cells and interneurons from rat neonatal hippocampal slices.
TLDR
It is demonstrated here that in neonatal (P2‐5) hippocampus, GABA is an excitatory neurotransmitter which can cause an elevation of [Ca2+]i in interneurons and pyramidal cells via activation of voltage‐dependent Ca2+ channels. Expand
Synchronization of neuronal activity in hippocampus by individual GABAergic interneurons
TLDR
It is demonstrated that individual GABAergic interneurons can effectively phase spontaneous firing and subthreshold oscillations in hippocampal pyramidal cells at 4–7 Hz, and this GABAergic mechanism is sufficient to synchronize the firing of pyramsidal cells. Expand
NMDA‐Dependent GABAA‐Mediated Polysynaptic Potentials in the Neonatal Rat Hippocampal CA3 Region
TLDR
Investigation of hippocampal neurons from brain slice preparations of rats revealed that the early phase arose from both direct and feedforward activation of GABAergic interneurons involving non‐NMDA receptors, while the late phase resulted from polysynaptic activation of neurons mediated by NMDA receptors. Expand
Excitatory actions of GABA in developing rat hypothalamic neurones.
TLDR
The data suggest that the mechanism for GABA‐induced depolarization is the depolarized Cl‐ reversal potential found in young but not older neurones, and the reversal potential of the GABA‐evoked current shifts negatively from depolarizing to hyperpolarizing in developing hypothalamus. Expand
Postnatal development of pre- and postsynaptic GABAB-mediated inhibitions in the CA3 hippocampal region of the rat.
TLDR
It is concluded that postsynaptic GABAB-mediated inhibition is absent or minimal during the first postnatal days in the CA3 region, and Baclofen reversibly reduced the amplitude of the evoked GABAA-mediated and glutamatergic synaptic events at all developmental stages. Expand
Modulation of GABA‐mediated Synaptic Potentials by Glutamatergic Agonists in Neonatal CA3 Rat Hippocampal Neurons
TLDR
It is suggested that, in early postnatal life, NMDA and non‐NMDA receptors located on GABAergic interneurons modulate GABAergic synaptic potentials. Expand
Modulation of GABA‐mediated synaptic transmission by endogenous zinc in the immature rat hippocampus in vitro.
TLDR
It is concluded that the naturally occurring large synaptic potentials in young CA3 neurones are apparently induced by endogenous Zn2+ which can promote or synchronize the release of GABA in the immature hippocampus. Expand
Synchronized oscillations in interneuron networks driven by metabotropic glutamate receptor activation
TLDR
It is proposed that interneuron network oscillations, in conjunction with intrinsic membrane resonances and long-loop (such as thalamocortical) interactions, contribute to 40-Hz rhythms in vivo. Expand
Intracellular correlates of hippocampal theta rhythm in identified pyramidal cells, granule cells, and basket cells
TLDR
Observations suggest that dendritic currents underlying theta in the awake rat may not be revealed under urethane anesthesia, and cellular‐synaptic generation of rhythmic slow activity in the hippocampus is investigated by intracellular recording from principal cells and basket cells in anesthetized rats. Expand
...
1
2
3
4
...