Synaptic vesicles form by budding from tubular extensions of sorting endosomes in PC12 cells.

@article{Wit1999SynapticVF,
  title={Synaptic vesicles form by budding from tubular extensions of sorting endosomes in PC12 cells.},
  author={H. G. M. de Wit and Y Lichtenstein and Hans J. Geuze and Regis B. Kelly and Peter van der Sluijs and Judith Klumperman},
  journal={Molecular biology of the cell},
  year={1999},
  volume={10 12},
  pages={
          4163-76
        }
}
The putative role of sorting early endosomes (EEs) in synaptic-like microvesicle (SLMV) formation in the neuroendocrine PC12 cell line was investigated by quantitative immunoelectron microscopy. By BSA-gold internalization kinetics, four distinct endosomal subcompartments were distinguished: primary endocytic vesicles, EEs, late endosomes, and lysosomes. As… CONTINUE READING