Synaptic proteins associate with a sub-set of lipid rafts when isolated from nerve endings at physiological temperature.

@article{Gil2006SynapticPA,
  title={Synaptic proteins associate with a sub-set of lipid rafts when isolated from nerve endings at physiological temperature.},
  author={Carles Gil and Roger Cub{\'i} and Juan Blasi and Jos{\'e} Aguilera},
  journal={Biochemical and biophysical research communications},
  year={2006},
  volume={348 4},
  pages={1334-42}
}
Although the high presence of cholesterol in nerve terminals is well documented, specific roles of this lipid in transmitter release have remained elusive. Since cholesterol is a highly enriched component in the membrane microdomains known as lipid rafts, it is probable that these domains are very important in synaptic function. The extraction of lipid rafts using Brij 98 at 37 degrees C avoids the formation of nonspecific membrane aggregates at low temperature, allowing the isolation of more… CONTINUE READING