Synaptic Activity of the AFD Neuron inCaenorhabditis elegans Correlates with Thermotactic Memory

@article{Samuel2003SynapticAO,
  title={Synaptic Activity of the AFD Neuron inCaenorhabditis elegans Correlates with Thermotactic Memory},
  author={Aravinthan D. T. Samuel and Ruwan A. Silva and Venkatesh N. Murthy},
  journal={The Journal of Neuroscience},
  year={2003},
  volume={23},
  pages={373 - 376}
}
Thermotactic behavior in Caenorhabditis elegans is sensitive to both a worm's ambient temperature (Tamb) and its memory of the temperature of its cultivation (Tcult). The AFD neuron is part of a neural circuit that underlies thermotactic behavior. By monitoring the fluorescence of pH-sensitive green fluorescent protein localized to synaptic vesicles, we measured the rate of the synaptic release of AFD in worms cultivated at temperatures between 15 and 25°C, and subjected to fixed, ambient… Expand
Chapter 11 – Thermosensory Learning in Caenorhabditis elegans
TLDR
The functional analogy between the thermotaxis neural circuit and human brain structure is proposed, which may help elucidation of the in-depth circuit operation of human brain. Expand
A diacylglycerol kinase modulates long-term thermotactic behavioral plasticity in C. elegans
TLDR
The DGK-3 diacylglycerol kinase is identified as a thermal memory molecule that regulates the rate of TS resetting by modulating the temperature range of synaptic output, but not temperature sensitivity, of the AFD thermosensory neurons. Expand
The role of the AFD neuron in C. elegans thermotaxis analyzed using femtosecond laser ablation
TLDR
The data supports a model in which the AFD neuron regulates a mechanism for generating cryophilic bias, and shows that severing the dendrites of sensory neurons in young adult worms permanently abolishes their sensory contribution without functional regeneration. Expand
The C. elegans Thermosensory Neuron AFD Responds to Warming
TLDR
It is shown here that the AFD neuron responds to warming, and is suggested that C. elegans provides an ideal model to genetically and physiologically reveal the molecular mechanism for sensation and memory of temperature information. Expand
Transfer at C. elegans Synapses
TLDR
The first characterization of transfer at central synapses in C.elegans is undertaken, showing that the synapse between AFD and AIY, the first stage in the thermotactic circuit, exhibits excitatory, tonic and graded release. Expand
Molecular biology of thermosensory transduction in C. elegans
TLDR
Thermotaxis in the nematode Caenorhabditis elegans is an ideal behavioral paradigm by which to address the molecular mechanism underlying thermosensory transduction, and molecular genetic analysis revealed that sensation of ambient temperature is mediated mainly by cyclic guanosine monophosphate signaling in thermosensing neurons. Expand
Neuronal substrates of complex behaviors in C. elegans.
TLDR
This work has identified gene products required for nervous system function and elucidated the molecular and neural bases of behaviors in the nematode Caenorhabditis elegans. Expand
Evaluation of the long-term memory for thermosensation regulated by neuronal calcium sensor-1 in Caenorhabditis elegans
TLDR
The thermotaxis tracking model is suitable for evaluating the LTM regulated by NCS-1, and can be employed for elucidating regulatory functions of specific genes or effects of stimuli on memory in C. elegans. Expand
Evaluation of the long-term memory for thermosensation regulated by neuronal calcium sensor-1 in Caenorhabditis elegans
To evaluate whether the thermotaxis tracking model is suitable for assessing long-term memory (LTM) in the nematode Caenorhabditis elegans. Animals were trained at 20 °C overnight in presence ofExpand
Neurophysiological methods in C. elegans: an introduction.
  • W. Schafer
  • Biology, Medicine
  • WormBook : the online review of C. elegans biology
  • 2006
TLDR
This chapter presents a compendium of protocols and technical reports on the current state of the art in C. elegans electrophysiology and neuroimaging, including methods for calcium imaging in intact animals, in situ electrical recording from neurons and muscle cells, and in vitro recording from cultured neurons and oocytes. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 26 REFERENCES
Neural regulation of thermotaxis in Caenorhabditis elegans
TLDR
A neural model is proposed in which regulation of the activities of the two interneurons in opposite directions, depending on the cultivation temperature, is essential for thermotaxis, and an amphid sensory neuron, AFD, is a major thermosensory neuron. Expand
Thermotaxis in Caenorhabditis elegans Analyzed by Measuring Responses to Defined Thermal Stimuli
TLDR
Analysis of the movements of individual worms responding to defined thermal gradients found evidence for a mechanism for migration down Thermal gradients that is active at temperatures above the cultivation temperature, and a mechanisms for isothermal tracking that isactive near the cultivationTemperature. Expand
Optical Imaging of Calcium Transients in Neurons and Pharyngeal Muscle of C. elegans
Electrophysiology and optical indicators have been used in vertebrate systems to investigate excitable cell firing and calcium transients, but both techniques have been difficult to apply inExpand
The LIM Homeobox Gene ceh-14 Confers Thermosensory Function to the AFD Neurons in Caenorhabditis elegans
TLDR
Misexpression of ceh-14 in chemosensory neurons can restore thermotactic behavior without impairing the chemosensor function, and thereby confers thermosensory function to neurons. Expand
Specification of Thermosensory Neuron Fate in C. elegans Requires ttx-1, a Homolog of otd/Otx
TLDR
It is shown that ttx-1 regulates all differentiated characteristics of the AFD neurons, suggesting an evolutionary link between thermosensation and phototransduction in C. elegans and the vertebrate photosensory circuit. Expand
Genetics of chemotaxis and thermotaxis in the nematode Caenorhabditis elegans.
  • I. Mori
  • Biology, Medicine
  • Annual review of genetics
  • 1999
Molecular genetic analysis of chemotaxis and theramotaxis in Caenorhabditis elegans has revealed the molecular bases of olfaction, taste, and thermosensation, which, in turn, has demonstrated thatExpand
UNC-13 is required for synaptic vesicle fusion in C. elegans
TLDR
The synaptic physiology of unc-13 mutants in the nematode C. elegans supports models in which UNC-13 mediates either fusion of vesicles during exocytosis or priming of vESicles for fusion. Expand
Ca2+ Signaling via the Neuronal Calcium Sensor-1 Regulates Associative Learning and Memory in C. elegans
TLDR
It is shown that the neuron-specific calcium sensor-1 (NCS-1) is essential for optimal IT, and proper calcium signaling via NCS-1 defines a novel pathway essential for associative learning and memory. Expand
The structure of the nervous system of the nematode Caenorhabditis elegans.
The structure and connectivity of the nervous system of the nematode Caenorhabditis elegans has been deduced from reconstructions of electron micrographs of serial sections. The hermaphrodite nervousExpand
Visualization of synaptic specializations in live C. elegans with synaptic vesicle protein-GFP fusions
  • M. Nonet
  • Medicine, Biology
  • Journal of Neuroscience Methods
  • 1999
TLDR
The cell biology underlying the sorting of synaptic vesicle proteins, trafficking of vesicles to terminals, and the development of presynaptic specializations is now more amenable to forward genetic analysis using these synaptic markers. Expand
...
1
2
3
...