Symbiont-mediated RNA interference in insects.

@article{Whitten2016SymbiontmediatedRI,
  title={Symbiont-mediated RNA interference in insects.},
  author={Miranda M A Whitten and Paul D. Facey and Ricardo del Sol and Lorena T Fern{\'a}ndez-Mart{\'i}nez and Meirwyn C Evans and Jacob J Mitchell and Owen Bodger and Paul J Dyson},
  journal={Proceedings. Biological sciences},
  year={2016},
  volume={283 1825},
  pages={
          20160042
        }
}
RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus… CONTINUE READING
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