Switching from HPLC/UV to MEIA for whole blood sirolimus quantitation: comparison of methods.

@article{Pini2006SwitchingFH,
  title={Switching from HPLC/UV to MEIA for whole blood sirolimus quantitation: comparison of methods.},
  author={Luigi Alberto Pini and Daniela Gallesi and Daria Brovia and Alfio Bertolini and Diego Pinetti and Valentina Ruggieri and Stefania Pisa and Brunella Poppi and Carmela Nives Castellana},
  journal={Journal of clinical laboratory analysis},
  year={2006},
  volume={20 6},
  pages={239-44}
}
Sirolimus is a immunosuppressive agent for renal transplant recipients. Monitoring of whole blood sirolimus concentration is necessary in order to improve clinical outcomes. An increasing number of clinical laboratories (4-14% during 2005) are using microparticle enzyme immunoassay (MEIA) for sirolimus quantitation but previous reports indicated a high variability, with a mean difference of 17% for MEIA method vs. high-performance liquid chromatography/ultraviolet (HPLC/UV). This study was… CONTINUE READING