Swiss 3T3 mouse embryo fibroblasts transfected with a human prepro-GRP gene synthesize and secrete pro-GRP rather than GRP.

@article{LebacqVerheyden1988Swiss3M,
  title={Swiss 3T3 mouse embryo fibroblasts transfected with a human prepro-GRP gene synthesize and secrete pro-GRP rather than GRP.},
  author={Anne-Marie Lebacq-Verheyden and Shraga Segal and Frank Cuttitta and James F. Battey},
  journal={Journal of cellular biochemistry},
  year={1988},
  volume={36 3},
  pages={
          237-48
        }
}
A prepro-gastrin-releasing peptide (GRP) gene was introduced into Swiss 3T3 mouse embryo fibroblasts by DNA transfection in an attempt to establish autocrine growth stimulation. Clonal transfectants expressed varying amounts of GRP encoding mRNA. They synthesized and secreted a approximately 15-kd pro-GRP hormone but not fully processed 2.8-kd GRP. Accordingly, no changes in growth properties were associated with GRP gene expression. We postulate that Swiss 3T3 fibroblasts lack the enzymes… CONTINUE READING
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