Swapping the domains of exoribonucleases RNase II and RNase R: conferring upon RNase II the ability to degrade ds RNA.

@article{Matos2011SwappingTD,
  title={Swapping the domains of exoribonucleases RNase II and RNase R: conferring upon RNase II the ability to degrade ds RNA.},
  author={Rute Gonçalves Matos and Ana Barbas and Paulino G{\'o}mez-Puertas and Cec{\'i}lia M Arraiano},
  journal={Proteins},
  year={2011},
  volume={79 6},
  pages={1853-67}
}
RNase II and RNase R are the two E. coli exoribonucleases that belong to the RNase II super family of enzymes. They degrade RNA hydrolytically in the 3' to 5' direction in a processive and sequence independent manner. However, while RNase R is capable of degrading structured RNAs, the RNase II activity is impaired by dsRNAs. The final end-product of these two enzymes is also different, being 4 nt for RNase II and 2 nt for RNase R. RNase II and RNase R share structural properties, including 60… CONTINUE READING