Sustained transgene expression in rat kidney with naked plasmid DNA and PCR-amplified DNA fragments.

@article{Maruyama2005SustainedTE,
  title={Sustained transgene expression in rat kidney with naked plasmid DNA and PCR-amplified DNA fragments.},
  author={Hiroki Maruyama and Noboru Higuchi and Shigemi Kameda and Gen Nakamura and Masaaki Shimotori and Noriaki Iino and Masato Higuchi and Tomohiro Neichi and Sadaaki Yokoyama and Toru Kono and Jun-ichi Miyazaki and Fumitake Gejyo},
  journal={Journal of biochemistry},
  year={2005},
  volume={137 3},
  pages={373-80}
}
Recently, we developed a kidney-targeted gene transfer technique, in which naked DNA was injected into the renal vein while the renal vein and artery were clamped. Kidney-targeted DNA transfer with only the renal vein clamped is an important modification that may permit less invasive catheter-based gene transfer in future clinical applications. The preparation of PCR-amplified DNA fragments is less time-consuming than that of naked plasmid DNA. We examined rat erythropoietin (Epo) plasmid… CONTINUE READING