Sustained exposure to high glucose concentrations modifies glucose signaling and the mechanics of secretory vesicle fusion in primary rat pancreatic beta-cells.

@article{Tsuboi2006SustainedET,
  title={Sustained exposure to high glucose concentrations modifies glucose signaling and the mechanics of secretory vesicle fusion in primary rat pancreatic beta-cells.},
  author={Takashi Tsuboi and Magalie A. Ravier and Laura E. Parton and Guy A Rutter},
  journal={Diabetes},
  year={2006},
  volume={55 4},
  pages={1057-65}
}
The mechanism(s) by which chronic hyperglycemia impairs glucose-stimulated insulin secretion is poorly defined. Here, we compare the "nanomechanics" of single exocytotic events in primary rat pancreatic beta-cells cultured for 48 h at optimal (10 mmol/l) or elevated (30 mmol/l) glucose concentrations. Cargo release was imaged by total internal reflection fluorescence microscopy of lumen-targeted probes (neuropeptide Y [NPY]-pH-insensitive yellow fluorescent protein [NPY-Venus] or NPY-monomeric… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 26 extracted citations

Insulin secretion from beta cells within intact islets: Location matters

Clinical and experimental pharmacology & physiology • 2015
View 2 Excerpts

References

Publications referenced by this paper.
Showing 1-10 of 53 references

GA: 5 -AMP-activated protein kinase controls insulin-containing secretory vesicle dynamics

T Tsuboi, G daSilvaXavier, I Leclerc, Rutter
J Biol Chem 278:52042–52051, • 2003
View 6 Excerpts
Highly Influenced

: insulin resistance and  - cell function

RA DeFronzo
2004

Direct imaging shows that insulin granule exocytosis occurs by complete vesicle fusion.

Proceedings of the National Academy of Sciences of the United States of America • 2004
View 1 Excerpt

Molecular Defects in Insulin Secretion in Type-2 Diabetes

Reviews in Endocrine and Metabolic Disorders • 2004
View 2 Excerpts

Similar Papers

Loading similar papers…