Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean

  title={Susceptibility to Agrobacterium tumefaciens and cotyledonary node transformation in short-season soybean},
  author={Pauline A. Donaldson and Daina H. Simmonds},
  journal={Plant Cell Reports},
 Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/pBI121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of… CONTINUE READING
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