Susceptibility of actin to modification by 4-hydroxy-2-nonenal.

  title={Susceptibility of actin to modification by 4-hydroxy-2-nonenal.},
  author={Munetaka Ozeki and Aya Miyagawa-Hayashino and Shinya Akatsuka and Tomoyuki Shirase and Wen-Hua Lee and Koji Uchida and Shinya Toyokuni},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  volume={827 1},
4-Hydroxy-2-nonenal (HNE), a major lipid peroxidation product, reacts with histidine, lysine or cysteine residues of proteins to form hemiacetal Michael adducts and thus interferes with the functions of the proteins. Here we undertook to identify HNE-modified proteins in the target organ of a ferric nitrilotriacetate (Fe-NTA)-induced renal carcinogenesis model with histidine-specific HNEJ-2 antibody. Immunoaffinity column separation and sequencing identified one of the major modified proteins… CONTINUE READING