Susceptibility of Scrapie Agent to Ionizing Radiation

@article{Field1969SusceptibilityOS,
  title={Susceptibility of Scrapie Agent to Ionizing Radiation},
  author={Ephraim Joshua Field and Faith Farmer and Ernest A. Caspary and G. Joseph Rahway Joyce},
  journal={Nature},
  year={1969},
  volume={222},
  pages={90-91}
}
THE infective agent of scrapie, a progressive encephalopathy endemic in sheep, has recently been the subject of much speculation. Its remarkable stability in a variety of physical and chemical conditions and in particular its resistance to ionizing radiation have given rise to a number of heterodox ideas as to the nature and mode of action of the agent1–3. The resistance of the scrapie agent to both ionizing and ultraviolet irradiation has led Alper and her colleagues4 to state that “it does… Expand
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The kinetic studies reported here demonstrate that scrapie's resistance to many inactivants is limited to small subpopulations of the total infectivity, the majority population being highly sensitive to inactivation. Expand
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TLDR
Scrapie is a fatal disease of the CNS that occurs naturally in sheep and goats and affected animals have either incoordinated movements, particularly in the hind limbs or show signs of intense pruritis. Expand
Pathogenesis of subacute spongiform encephalopathies.
TLDR
Experimental studies of scrapie after subcutaneous inoculation demonstrated early replication of the agent in lymphoid tissues and later appearance in other organs; as the amount of agent in the central nervous system increased, it decreased in or disappeared from lymphoid tissue. Expand
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TLDR
It is suggested tentatively that the scrapie agent consists of a small nucleic acid core enclosed in a mucopolysaccharide or even polysaccharides coat, and further that the nucleic acids core is stabilized structurally by covalent bonds with the sugar residues in the coat. Expand
Nature of the Scrapie Agent
TLDR
It is suggested that scrapie can best be considered to arise from a replicable change in the structural pattern of a commonly occurring unit membrane and three possible mechanisms for its self-replication are proposed. Expand
The infective process in scrapie.
Abstract It is suggested that the essential part of the scrapie-infection process is the transfer, from infected host, of a " linkage substance " which enables pathologically incompetent, incompleteExpand
Does the Agent of Scrapie Replicate without Nucleic Acid ?
TLDR
Experiments on the effects of ultra-violet irradiation of suspensions of infected mouse brain extracts confirm that the agent responsible for scrapie does not depend on a nucleic acid for its ability to replicate, but no evidence is obtained of whether the agent is associated with a protein. Expand
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SUMMARY: Saline extracts of mouse-scrapie brain homogenates lost little infectivity when heated at 75° for one hour. At higher temperatures, there was a progressive inactivation of the agent,Expand
Protection by anoxia of the scrapie agent and some DNA and RNA viruses irradiated as dry preparations.
TLDR
Freeze-dried preparations of the scrapie agent and of 5 strains of virus were used to determine the extent of protection afforded against ionizing radiation by anoxia, finding no obvious correlation of oxygen enhancement ratio with type or size of nucleic acid core. Expand
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During the search for an intracellular form of N1 phage DNA in infected M. lysodeikticus cells, the existence of small closed circular DNA molecules (which the authors call minicircles) in uninfected as well as infected cells is discovered. Expand
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