Susceptibility of Cryptococcus neoformans biofilms to antifungal agents in vitro.

@article{Martnez2006SusceptibilityOC,
  title={Susceptibility of Cryptococcus neoformans biofilms to antifungal agents in vitro.},
  author={Luis River{\'o}n Mart{\'i}nez and Arturo Casadevall},
  journal={Antimicrobial agents and chemotherapy},
  year={2006},
  volume={50 3},
  pages={1021-33}
}
Microbial biofilms contribute to virulence and resistance to antibiotics by shielding microbial cells from host defenses and antimicrobial drugs, respectively. Cryptococcus neoformans was demonstrated to form biofilms in polystyrene microtiter plates. The numbers of CFU of disaggregated biofilms, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide reduction, and light and confocal microscopy were used to measure the fungal mass, the metabolic activity… CONTINUE READING