Survival of mouse morulae vitrified in an ethylene glycol-based solution after exposure to the solution at various temperatures.

@article{Kasai1992SurvivalOM,
  title={Survival of mouse morulae vitrified in an ethylene glycol-based solution after exposure to the solution at various temperatures.},
  author={Magosaburo Kasai and Mina Nishimori and Shi En Zhu and Toshihiro Sakurai and Takashi Machida},
  journal={Biology of reproduction},
  year={1992},
  volume={47 6},
  pages={
          1134-9
        }
}
Mouse morulae were exposed in one step to a vitrification solution (EFS, a modified PBS containing 40% ethylene glycol, 18% Ficoll, and 0.3-M sucrose) at various temperatures, then cooled rapidly in liquid nitrogen, and then warmed rapidly. All of the embryos exposed to the EFS solution for 0.5 min at 25 degrees C before vitrification developed in culture. However, survival rates were lower if the duration of exposure was prolonged to 2, 5, or 10 min. At lower ambient temperatures (20, 10, and… CONTINUE READING
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