Survival of mint shoot tips after exposure to cryoprotectant solution components.

  title={Survival of mint shoot tips after exposure to cryoprotectant solution components.},
  author={Gayle M. Volk and Jackie L Harris and Kate E Rotindo},
  volume={52 2},
Many plant species can be cryopreserved by treating shoot tips with complex cryoprotectant solutions before rapidly cooling them to liquid nitrogen temperatures. Plant vitrification solution 2 (PVS2), a commonly selected cryoprotectant, can be lethal with extended exposure times. To determine potentially toxic combinations, we have exposed mint shoot tips to one-, two-, three-, and four-component solutions of PVS2 chemicals (30% glycerol, 15% ethylene glycol, 15% dimethyl sulfoxide, and 0.4 M… CONTINUE READING


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