Survival of frozen-thawed human red cells as a function of cooling and warming velocities.

@article{Miller1976SurvivalOF,
  title={Survival of frozen-thawed human red cells as a function of cooling and warming velocities.},
  author={Rebecca H Miller and Peter Mazur},
  journal={Cryobiology},
  year={1976},
  volume={13 4},
  pages={404-14}
}
Human red cells were equilibrated for 30 min at 20degreesC in buffered saline containing 2 M glycerol and then frozen to --196degreesC at 0.27, 1.7, 59, 180, 480, 600, and 1300degreesC/min and warmed at 0.47, 1, 26, 160, and 550degreesC/min. Cells frozen at 600 and 1300degreesC/min responded in the classical fashion for cells containing intracellular ice; i.e., survivals were low when warming was slow (less than 10%), but increased progressively with increasing warming rate. The sensitivity to… CONTINUE READING