Survival of frozen mouse embryos after rapid thawing from -196 degrees C.

@article{Whittingham1979SurvivalOF,
  title={Survival of frozen mouse embryos after rapid thawing from -196 degrees C.},
  author={David G Whittingham and Margaret Wood and John Farrant and H Lee and J A Halsey},
  journal={Journal of reproduction and fertility},
  year={1979},
  volume={56 1},
  pages={11-21}
}
The effect of the rate of rewarming on the survival of 8-cell mouse embryos and blastocysts was examined. The samples were slowly cooled (0.3--0.6 degrees C/min) in 1.5 M-DMSO to temperatures between -10 and -80 degrees C before direct transfer to liquid nitrogen (-196 degrees C). Embryos survived rapid thawing (275--500 degrees C/min) only when slow cooling was terminated at relatively high subzero temperatures (-10 to -50 degrees C). The highest levels of survival in vitro of rapidly thawed 8… CONTINUE READING

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