Survival and viability of nonculturableEscherichia coli andVibrio cholerae in the estuarine and marine environment

@article{Xu1982SurvivalAV,
  title={Survival and viability of nonculturableEscherichia coli andVibrio cholerae in the estuarine and marine environment},
  author={Huai -Shu Xu and Neil Roberts and F. L. Singleton and R. W. Attwell and Darrell Jay Grimes and Rita R. Colwell},
  journal={Microbial Ecology},
  year={1982},
  volume={8},
  pages={313-323}
}
Plating methods for estimating survival of indicator organisms, such asEscherichia coli, and water-borne pathogens includingVibrio cholerae, have severe limitations when used to estimate viable populations of these organisms in the aquatic environment. By combining the methods of immunofluorescent microscopy, acridine orange direct counting, and direct viable counting, with culture methods such as indirect enumeration by most probable number (MPN) estimation and direct plating, it was shown… CONTINUE READING
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