Survival and behavior of Onchocerca gutturosa microfilariae in vitro.

  title={Survival and behavior of Onchocerca gutturosa microfilariae in vitro.},
  author={S S McDiarmid and Bernard E. Matthews},
  journal={The Journal of parasitology},
  volume={68 3},
Microfilariae of Onchocerca gutturosa were extracted from the skin of the bovine naval by teasing in saline and then suspending the skin in a gauze bag overnight in Tyrode's solution with 20% human serum. They survived for ten days in this medium, with antibiotics, in plastic microtitration plates at 11 C. After dispersal in flat containers, microfilariae slowly assumed clumped dispersion patterns. They also accumulated in glass wool, suggesting that aggregation was a tactile phenomenon… CONTINUE READING

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