Survival and Adaptation of the Thermophilic Species Geobacillus thermantarcticus in Simulated Spatial Conditions

  title={Survival and Adaptation of the Thermophilic Species Geobacillus thermantarcticus in Simulated Spatial Conditions},
  author={Paola Di Donato and Ida Romano and Vincenza Mastascusa and Annarita Poli and Pierangelo Orlando and Mariagabriella Pugliese and Barbara Nicolaus},
  journal={Origins of Life and Evolution of Biospheres},
Astrobiology studies the origin and evolution of life on Earth and in the universe. According to the panspermia theory, life on Earth could have emerged from bacterial species transported by meteorites, that were able to adapt and proliferate on our planet. Therefore, the study of extremophiles, i.e. bacterial species able to live in extreme terrestrial environments, can be relevant to Astrobiology studies. In this work we described the ability of the thermophilic species Geobacillus… 

Peculiarities and biotechnological potential of environmental adaptation by Geobacillus species

  • Hirokazu Suzuki
  • Biology, Engineering
    Applied Microbiology and Biotechnology
  • 2018
This phenomenon provides a new approach to generate thermostable enzymes, termed as thermoadaptation-directed enzyme evolution, thereby expanding the biotechnological potentials of Geobacillus spp.

Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure

This study investigates the resistance of spores of the thermophilic bacteria Parageobacillus thermantarcticus to γ-rays by means of Raman spectroscopy, allowing to highlight the mechanism(s) of inactivation including DNA damage, protein denaturation and calcium dipicolinate levels.

Resistance to high temperatures of spores from bacilli of shallow hydrothermal vent origins

Thermophiles from shallow hydrothermal vents (SHV) are ideal candidates to extend our knowledge for understanding the environmental limits for terrestrial life, which are also relevant in the field

Genomic Analysis Provides New Insights Into Biotechnological and Industrial Potential of Parageobacillus thermantarcticus M1

Genetic analysis of P. thermantarcticus M1 pointed out the significant potential of strain M1 in various biotechnological and industrial applications considering its capacity to produce EPSs, ethanol and thermostable enzymes via the utilization of lignocellulosic waste materials.

Effects of Ionizing Radiation and Long-Term Storage on Hydrated vs. Dried Cell Samples of Extremophilic Microorganisms

The results reported here suggest that the selected extremophilic microorganisms could serve as biological model for space simulation and/or real space condition exposure, since they showed good resistance to ionizing radiation exposure and were able to resume cellular growth after long-term storage.

Transcript Regulation of the Recoded Archaeal α-l-Fucosidase In Vivo

Analysis of the transcription of this recoded archaeal α-l-fucosidase and of its full-length mutant in different growth conditions in vivo indicated that the increased level of fucA mRNA cannot be explained by transcript up-regulation alone and a different mechanism related to translation efficiency is discussed.

Graphene-Based and Surface-Enhanced Raman Spectroscopy for Monitoring the Physio-Chemical Response of Thermophilic Bacterial Spores to Low Temperatures Exposure

The results obtained showed that the joint use of Graphene-Based Raman Spectroscopy (GbRS) and SERS represents a valuable tool for monitoring the physio-chemical response of bacterial spores upon exposure to stressing stimuli.

Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments

One isolate, Pseudomonas extremaustralis strain 2ASCA, showed the capability to produce, in the loosely bound cell fraction, a levan-type polysaccharide with a yield of 613 mg/L of culture, suggesting its suitability as a candidate for eco-sustainable alternatives to commercial polymers.

Parageobacillus thermantarcticus, an Antarctic Cell Factory: From Crop Residue Valorization by Green Chemistry to Astrobiology Studies

Parageobacillus thermantarcticus strain M1 was isolated from geothermal soil of the crater of Mount Melbourne during the Italian Antarctic Expedition and the recent acquisition of its genome sequence opens up new opportunities for the use of this versatile bacterium in still unexplored biotechnology sectors.



Pb2+ Effects on Growth, Lipids, and Protein and DNA Profiles of the Thermophilic Bacterium Thermus Thermophilus

In this work Thermus thermophilus was challenged against increasing Pb2+ concentrations spanning from 0 to 300 ppm in order to ascertain the sensitiveness of this bacteria to the Pb environmental pollution and to give an insight on its heavy metal resistance mechanisms.

Extremophiles Survival to Simulated Space Conditions: An Astrobiology Model Study

Examining the ability of four extremophilic bacteria from Archaea and Bacteria domains to resist to space environment by exposing them to extreme conditions of temperature, UV radiation, desiccation coupled to low pressure generated in a Mars’ conditions simulator showed that H. hispanica and G. thermantarcticus were resistant to desICcation and low pressure.

The Survival and Resistance of Halobacterium salinarum NRC-1, Halococcus hamelinensis, and Halococcus morrhuae to Simulated Outer Space Solar Radiation.

Overall, this study shows that some halophilic archaea are highly resistant to simulated solar radiation and that they are of high astrobiological significance.

Venturing into new realms? Microorganisms in space.

Information on the microbiology of the ISS will have an impact on the planning and implementation of long-term human spaceflights in order to ensure a safe, stable and balanced microbiome on board.

Space Microbiology

The data support the likelihood of interplanetary transfer of microorganisms within meteorites, the so-called lithopanspermia hypothesis.

Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy

Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AF/04T falls within the genus Anoxybacillus, a novel aerobe thermophilic endospore-forming bacterium isolated from thermal mud located in Euganean hot springs, Abano Terme, Padova, Italy.

Protection of Bacterial Spores in Space, a Contribution to the Discussion on Panspermia

The data suggest that in a scenario of interplanetary transfer of life, small rock ejecta of a few cm in diameter could be sufficiently large to protect bacterial spores against the intense insolation; however, micron-sized grains, as originally requested by Panspermia, may not provide sufficient protection for spores to survive.

Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica.

A Gram-stain-positive, non-endospore-forming, haloalkaliphilic actinobacterium, strain CK5T, was isolated from a soil sample, collected at Cape King (Antarctica), and its taxonomic position was

Geobacillus galactosidasius sp. nov., a new thermophilic galactosidase-producing bacterium isolated from compost.