Survival, morphology and adhesion properties of cerebellar interneurones cultured in chemically defined and serum-supplemented medium.

@article{Kingsbury1985SurvivalMA,
  title={Survival, morphology and adhesion properties of cerebellar interneurones cultured in chemically defined and serum-supplemented medium.},
  author={Ann E Kingsbury and V. Gallo and Peter L. Woodhams and R{\'o}bert Bal{\'a}zs},
  journal={Brain research},
  year={1985},
  volume={349 1-2},
  pages={17-25}
}
Cultures obtained from early postnatal rat cerebellum, grown in either chemically defined or in serum-supplemented medium containing 25 mM K+, contained predominantly (greater than 90%) small interneurones, mostly granule cells, with good and comparable viability (assessed by the retention of preloaded 51Cr). Neuronal survival was prolonged in the chemically defined medium, nerve cells living up to two weeks longer than in serum-supplemented medium, although the proportion of non-neuronal cells… CONTINUE READING

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