Survey of Catalytic Residues and Essential Roles of Glutamate-170 and Aspartate-335 in Coenzyme B 12-dependent Diol Dehydratase *

The importanceof eachactive-site residue inadenosylcobalamindependent diol dehydratase of Klebsiella oxytoca was estimated usingmutant enzymes in which one of the residues interacting with substrate and/or K was mutated to Ala or another amino acid residue. The E 170A and D 335A mutants were totally inactive, and the H 143Amutant showed only a trace of… (More)