Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media

@article{Hama2010SurfactantmodifiedYW,
  title={Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media},
  author={Shinji Hama and Ayumi Yoshida and Kazunori Nakashima and Hideo Noda and Hideki Katano-shi Fukuda and Akihiko Kondo},
  journal={Applied Microbiology and Biotechnology},
  year={2010},
  volume={87},
  pages={537-543}
}
The cell surface engineering system, in which functional proteins are genetically displayed on microbial cell surfaces, has recently become a powerful tool for applied biotechnology. Here, we report on the surfactant modification of surface-displayed lipase to improve its performance for enzymatic synthesis reactions. The lipase activities of the surfactant-modified yeast displaying Rhizopus oryzae lipase (ROL) were evaluated in both aqueous and nonaqueous systems. Despite the similar lipase… CONTINUE READING
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