Surface staining of cartilage by Alcian Blue, with reference to the role of microscopic dye aggregates in histological staining

@article{Goldstein1974SurfaceSO,
  title={Surface staining of cartilage by Alcian Blue, with reference to the role of microscopic dye aggregates in histological staining},
  author={David John Goldstein and Richard W. Horobin},
  journal={The Histochemical Journal},
  year={1974},
  volume={6},
  pages={175-184}
}
Anisotropic, highly reflecting dye particles of microscopic size are visible in epithelial mucin stained with Alcian Blue. The formation of these exceptionally large dye deposits is attributed to the inherently high tendency of Alcian Blue to aggregate, together with the extremely porous and permeable character of hydrated mucin which allows the growth of dye crystalsin situ. Other basophilic tissue components may be too dense to permit a similar interstitial dye crystal growth. Alcian Blue and… CONTINUE READING

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