Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations

@article{Jondal1975SurfaceMO,
  title={Surface markers on human b and t lymphocytes. VI. Cytotoxicity against cell lines as a functional marker for lymphocyte subpopulations},
  author={Mikael Jondal and Hugh F. Pross},
  journal={International Journal of Cancer},
  year={1975},
  volume={15}
}
  • M. Jondal, H. Pross
  • Published 15 April 1975
  • Biology, Medicine
  • International Journal of Cancer
The spontaneous lymphocyte‐mediated cytotoxicity (SLMC) of cells from normal donors against 19 different established cell lines was analysed. All normal lymphocytes were cytotoxic in all combinations tested in a 17 h 51Cr release assay. SLMC was found to be mediated by a minor subpopulation of lymphocytes with Fc/C3 receptors. Noncytotoxic SRBC binding T‐lymphocytes could be induced to become cytotoxic by the addition of Con A to the incubation medium. SLMC and lectin‐induced cytotoxicity were… 
Requirement for monocytes in the spontaneous cytotoxic effects of human lymphocytes against non-lymphoid target cells
TLDR
Evidence is presented that monocytes have a helper function in the lymphocyte-mediatedSCMC against target cells growing in monolayer cultures, whereas effector cells in SCMC against lymphoid target cellsgrowing in suspension cultures were lysed by lymphocytes in the absence of monocytes.
Human spontaneous lymphocyte-mediated cytotoxicity (SLMC) against malignant and normal tissue-derived target cell lines tested in autologous and allogeneic combinations by the microcytotoxicity assay
TLDR
Effector cell types and effector mechanisms of human spontaneous lymphocyte-mediated cytotoxicity (SLMC) were studied in a 44-h microcytotoxicity titration assay which registered lymphocyte/target cell interactions which differed with regard to specificity, effector cell type, and immunoglobulin dependency.
Natural cytotoxicity in man: activity of lymph node and tumor‐infiltrating lymphocytes
TLDR
Tests with blood taken during operation showed that the lack of reactivity in these preparations was unlikely to be a result of the effects of anesthesia or surgery, and the reason for the low cytotoxicity in the lymph node and tumor‐infiltrating lymphocytes is as yet undefined.
Effector Cell Potential of Two Human T‐Cell Subpopulations with Different Affinities to Sheep Erythrocytes
TLDR
Two human T lymphocyte Subpopulations were purified on the basis of their different affinities to sheep erythrocytes and compared with unseparated and non‐T lymphocytes as effectors in cell‐mediated lymphoksis and addition of human leucocyte interferon to the ADCC and SCMC cultures resulted in enhanced SCMC.
Spontaneous human lymphocyte‐mediated cytotoxicity againts tumour target cells. I. The effect of malignant disease
TLDR
Lymphocytes from all normal donors tested showed significant activity in the SLMC assay, with some variation from day to day, and lymphocytes from many patients with malignant disease had decreased SLM activity, and this decrease was highly significant in patients with treated or untreated metastatic disease, or untreated CLL.
Stimulation of Normal Lymphocytes with Autologous Lymphoid Cell Lines: Properties of Derived Killer Cells
TLDR
The sensitivity of target cells to lysis correlated positively with their capacity to block AS lymphocyte lysis of autologous LCLs in competition experiments, and the cytotoxicity of AS lymphocytes was blocked by EBV‐genome‐positive and‐negative cell lines, whereas theEBV‐related cytot toxicity of T cells from acute cases of infectious mononucleosis was blockedBy EBV•genome-positive LCL only.
Induction of Natural Killer Cell Activity and Allocytotoxicity in Human Peripheral Blood Lymphocytes after Mixed Lymphocyte Culture
TLDR
It was demonstrated that fresh NK cells lose their NK lytic activity when cultured alone and there is no antigenic relationship between the recognition sites for the alloreactive T lymphocytes and MLC‐generated NK cells expressed on the lymphoid target cells and the tumour target cells, respectively.
Spontaneous human lymphocyte‐mediated cytotoxicity against tumor target cells. II. Is the complement receptor necessarily present on the killer cells?
TLDR
No direct evidence was found to support a role for T cells in SLMC, although E rosette depletion resulted in lower than expected cytotoxicity in view of the number of Fc receptor‐bearing cells remaining in the preparation.
Heterogeneity among human lymphocyte effector cells mediating spontaneous lymphocyte‐mediated cytotoxicity
TLDR
The results indicate that effector cells mediating SLMC are a heterogeneous lymphocyte population composed of Fc‐receptor‐positive lymphocytes both with and without C3 receptors that consistently segregate with cells that do not form rosettes with sheep red blood cells.
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References

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Surface Markers on Human B and T Lymphocytes
  • M. Jondal
  • Biology, Medicine
    Scandinavian journal of immunology
  • 1974
TLDR
It is argued that caution must be taken when these receptors are used as B‐cell markers because of the fact that Fc/C3 receptors were lacking on almost all B blast cells, as well as for other reasons elaborated in the text.
SURFACE MARKERS ON HUMAN B AND T LYMPHOCYTES
TLDR
Human peripheral lymphocytes were investigated for receptors binding Epstein-Barr virus because of the regular association of this virus with infectious mononucleosis and Burkitt's lymphoma and it was concluded that B lymphocytes, in contrast to T lymphocyte, have receptors for EBV.
Cytotoxic effector cells specific for B Cell lines transformed by Epstein-Barr virus are present in patients with infectious mononucleosis.
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  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1975
TLDR
Results indicate that, during the acute phase of this disease, patients harbor killer cells, probably T cells, which specifically kill EBV-genome-carrying B cells in vitro.
Cell‐Mediated Lympholysis after Sensitization in Unidirectional Mixed Lymphocyte Culture in Man
The cytotoxicity of human peripheral blood lymphocytes (PBL), after a 6‐day allogeneic stimulation in unidirectional mixed lymphocyte culture (UMLC). was studied. PBLs stimulated with
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TLDR
Evidence indicating antigenic cross‐reactivity between tumors of the same histological type was obtained for the following seven groups of neoplasms: malignant melanomas, carcinomas of the colon, breast, testis, endometrium and ovary, and various sarcomas.
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TLDR
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TLDR
Immunological and physical characterization of the effector cells generated in MLC-Imm indicated that they were medium to large-sized T lymphocytes, suggesting the existence of an anamnestic cell-mediated cytotoxic response in M LC-Imm.
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TLDR
The notion that the effector cells in cell-mediated immunity to carcinoma of the urinary bladder are members of the nonthymus-derived population of peripheral lymphocytes is supported.
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TLDR
The weak in vitro reactivity of the lymphocytes in Hodgkin's disease is in line with the view that deficient lymphocytes are present in this disease and suggests that the patients' pool of immunologically competent cells is reduced.
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