Surface-layer protein from Caulobacter crescentus: expression, purification and X-ray crystallographic analysis.

@article{Jones2016SurfacelayerPF,
  title={Surface-layer protein from Caulobacter crescentus: expression, purification and X-ray crystallographic analysis.},
  author={Michael D. Jones and Anson C. K. Chan and John F. Nomellini and Michael E P Murphy and John Smit},
  journal={Acta crystallographica. Section F, Structural biology communications},
  year={2016},
  volume={72 Pt 9},
  pages={677-80}
}
Protein surface layers are self-assembling, paracrystalline lattices on the surface of many prokaryotes. Surface-layer proteins have not benefited from widespread structural analysis owing to their resistance to crystallization. Here, the successful expression of a truncated version of RsaA, the surface-layer protein from Caulobacter crescentus, from a… CONTINUE READING