Surface fucosylation of human cord blood cells augments binding to P-selectin and E-selectin and enhances engraftment in bone marrow.

@article{Xia2004SurfaceFO,
  title={Surface fucosylation of human cord blood cells augments binding to P-selectin and E-selectin and enhances engraftment in bone marrow.},
  author={Lijun Xia and John Michael Mcdaniel and Tadayuki Yago and Andrea Doeden and Rodger P. McEver},
  journal={Blood},
  year={2004},
  volume={104 10},
  pages={
          3091-6
        }
}
Murine hematopoietic stem and progenitor cells (HSPCs) home to bone marrow in part by rolling on P-selectin and E-selectin expressed on endothelial cells. Human adult CD34(+) cells, which are enriched in HSPCs, roll on endothelial selectins in bone marrow vessels of nonobese diabetic/severe combined immune deficiency (NOD/SCID) mice. Many human umbilical cord blood (CB) CD34(+) cells do not roll in these vessels, in part because of an uncharacterized defect in binding to P-selectin. Selectin… 
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Enforced fucosylation of cord blood hematopoietic cells accelerates neutrophil and platelet engraftment after transplantation.
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Findings support ex vivo fucosylation of multipotent CD34(+) CB cells as a clinically feasible means to improve engraftment efficiency in the double CBT setting.
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It is established that the HCELL glycoform of CD44 confers tropism to bone and unveil a readily translatable roadmap for programming cellular trafficking by chemical engineering of glycans on a distinct membrane glycoprotein.
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Ex vivo expansion of fucosylated cells placed on the selectin‐coated scaffold serve as a basal surface for cell–cell interaction and more homing potential of uHSCs and can be considered as a promising technique for the hematological disorder treatment and tissue engineering applications.
Human Umbilical Cord Blood‐Derived Mesenchymal Stromal Cells Display a Novel Interaction between P‐Selectin and Galectin‐1
TLDR
It is discovered that P‐selectin (CD62P) binds to umbilical cord blood (UCB)‐derived MSCs independently of the previously known sialyl Lewis x (sLex)‐containing ligands such as P‐ selectin glycoprotein ligand‐1 (PSGL‐1, CD162).
The Expanding Family of Bone Marrow Homing Factors for Hematopoietic Stem Cells: Stromal Derived Factor 1 Is Not the Only Player in the Game
TLDR
A more complex picture of homing emerges that involves several factors supporting, and in some situations even replacing, the SDF-1-CXCR4 axis.
Glycoengineering of E‐Selectin Ligands by Intracellular versus Extracellular Fucosylation Differentially Affects Osteotropism of Human Mesenchymal Stem Cells
TLDR
This study represents the first direct analysis of E‐selectin ligand expression programmed on human MSCs by FTVI‐mediated intracellular versus extracellular fucosylation and the observed differential biologic effects of F TVI activity in these two contexts may yield new strategies for improving the efficacy of human M SCs in clinical applications.
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References

SHOWING 1-10 OF 51 REFERENCES
Functional selectin ligands mediating human CD34(+) cell interactions with bone marrow endothelium are enhanced postnatally.
TLDR
It is shown that endothelial selectins are necessary for human adult CD34(+) cell homing, since rolling on BM endothelium and retention in the BM compartment are drastically reduced (>90%) in endothelialselectin-deficient NOD/SCID mice.
Functional selectin ligands mediating human CD34(+) cell interactions with bone marrow endothelium are enhanced postnatally.
TLDR
It is shown that endothelial selectins are necessary for human adult CD34(+) cell homing, since rolling on BM endothelium and retention in the BM compartment are drastically reduced (>90%) in endothelialselectin-deficient NOD/SCID mice.
Stromal derived factor-1-induced chemokinesis of cord blood CD34(+) cells (long-term culture-initiating cells) through endothelial cells is mediated by E-selectin.
TLDR
To define the mechanism of constitutive expression of E-selectin by the BMEC in vivo, it is found that vascular endothelial growth factor (VEGF(165)) induces E- selectin expression by cultured endothelial cells.
Stromal derived factor-1-induced chemokinesis of cord blood CD34(+) cells (long-term culture-initiating cells) through endothelial cells is mediated by E-selectin.
TLDR
It is found that vascular endothelial growth factor (VEGF 165 ) induces E-selectin expression by cultured endothelial cells and suggests that trafficking of subsets of CD34 + cells with LTC-IC potential is determined in part by sequential interactions with E- selectin and SDF-1.
Adhesion to E-selectin promotes growth inhibition and apoptosis of human and murine hematopoietic progenitor cells independent of PSGL-1.
TLDR
It is demonstrated that adhesion to E-selectin inhibits the proliferation of human CD34+ cells isolated either from human umbilical cord blood, adult mobilized blood, or steady-state bone marrow.
PSGL-1 participates in E-selectin-mediated progenitor homing to bone marrow: evidence for cooperation between E-selectin ligands and alpha4 integrin.
TLDR
The results underscore a major difference between mature myeloid cells and immature stem/progenitor cells in that E-selectin ligands cooperate with alpha4 integrin rather than P-selectIn ligands.
Constitutive expression of E-selectin and vascular cell adhesion molecule-1 on endothelial cells of hematopoietic tissues.
TLDR
It is suggested that E-selectin plays a role in the homing of HPCs and that its constitutive expression on endothelial cells of hematopoietic organs may be essential in the initial step of the honing process.
Endothelial selectins and vascular cell adhesion molecule-1 promote hematopoietic progenitor homing to bone marrow.
TLDR
Results indicate that endothelial selectins play an important role in HPC homing to the BM, and that Optimal recruitment of HPC after lethal doses of irradiation requires the combined action of both selectins and VCAM-1 expressed on endothelium of the BM.
PSGL-1-mediated adhesion of human hematopoietic progenitors to P-selectin results in suppression of hematopoiesis.
Hematopoietic Progenitor Cell Rolling in Bone Marrow Microvessels: Parallel Contributions by Endothelial Selectins and Vascular Cell Adhesion Molecule 1
TLDR
BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions of hematopoietic progenitor cells, and a combination of constitutively expressed adhesion molecules may constitute a BM-specific recruitment pathway for progenitors cells analogous to the vascular addressins that direct selective lymphocyte homing to lymphoid organs.
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