Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

@article{Saha2011SurfaceengineeredSF,
  title={Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions},
  author={K. Saha and Ying Mei and Colin M Reisterer and N. Pyzocha and J. Yang and J. Muffat and M. Davies and M. Alexander and R. Langer and D. Anderson and R. Jaenisch},
  journal={Proceedings of the National Academy of Sciences},
  year={2011},
  volume={108},
  pages={18714 - 18719}
}
  • K. Saha, Ying Mei, +8 authors R. Jaenisch
  • Published 2011
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences
  • The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human pluripotent stem cell culture. Chemical and geometrical optimization of the surfaces enables control of early cell aggregation from fully dissociated cells, as predicted from a numerical model of cell… CONTINUE READING
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    References

    SHOWING 1-10 OF 46 REFERENCES
    Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells
    • 448
    • PDF
    Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells
    • 376
    Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511
    • 463
    Chemically defined conditions for human iPS cell derivation and culture
    • 969
    • PDF
    An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells.
    • 147
    • PDF
    A defined glycosaminoglycan-binding substratum for human pluripotent stem cells
    • 220
    • PDF
    Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin.
    • 124
    • PDF