Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

  title={Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions},
  author={K. Saha and Ying Mei and Colin M Reisterer and N. Pyzocha and J. Yang and J. Muffat and M. Davies and M. Alexander and R. Langer and D. Anderson and R. Jaenisch},
  journal={Proceedings of the National Academy of Sciences},
  pages={18714 - 18719}
  • K. Saha, Ying Mei, +8 authors R. Jaenisch
  • Published 2011
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences
  • The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human pluripotent stem cell culture. Chemical and geometrical optimization of the surfaces enables control of early cell aggregation from fully dissociated cells, as predicted from a numerical model of cell… CONTINUE READING
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