Surface display of monkey metallothionein α tandem repeats and EGFP fusion protein on Pseudomonas putida X4 for biosorption and detection of cadmium

Abstract

Monkey metallothionein α domain tandem repeats (4mMTα), which exhibit high cadmium affinity, have been displayed for the first time on the surface of a bacterium using ice nucleation protein N-domain (inaXN) protein from the Xanthomonas campestris pv (ACCC—10049) as an anchoring motif. The shuttle vector pIME, which codes for INAXN–4mMTα–EGFP fusion, was… (More)
DOI: 10.1007/s00253-011-3768-3

Topics

9 Figures and Tables