Surface charge control of electropermeabilization and glycophorin electroinsertion with 1,2-diacyl-sn-glycero-3-phosphocholine (lecithin) liposomes.

@article{Raffy1997SurfaceCC,
  title={Surface charge control of electropermeabilization and glycophorin electroinsertion with 1,2-diacyl-sn-glycero-3-phosphocholine (lecithin) liposomes.},
  author={Sophie Raffy and Justin Teissi{\'e}},
  journal={European journal of biochemistry},
  year={1997},
  volume={250 2},
  pages={315-9}
}
Back insertion of a solubilized membrane protein, glycophorin A, has been obtained in lipid multilamellar vesicles by applying calibrated electric field pulses on a lipid/protein mixture. Experimental evidence for insertion is given by means of immunofluorescence. Insertion was obtained only under field conditions that induced the leakage of a soluble hydrophilic molecule, calcein, which was trapped between the lipid layers. Studies were performed on mixed liposomes where charged species were… CONTINUE READING