Surface-bound optical probes monitor protein translocation and surface potential changes during the bacteriorhodopsin photocycle.

@article{Heberle1992SurfaceboundOP,
  title={Surface-bound optical probes monitor protein translocation and surface potential changes during the bacteriorhodopsin photocycle.},
  author={Joachim Heberle and Norbert A. Dencher},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 13},
  pages={
          5996-6000
        }
}
Light-induced H+ release and reuptake as well as surface potential changes inherent in the bacterio-rhodopsin reaction cycle were measured between 10 degrees C and 50 degrees C. Signals of optical pH indicators covalently bound to Lys-129 at the extracellular surface of bacteriorhodopsin were compared with absorbance changes of probes residing in the aqueous bulk phase. Only surface-bound indicators monitor the kinetics of H+ ejection from bacteriorhodopsin and allow the correlation of the… CONTINUE READING

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