Surface area regulation: underexplored yet crucial in cell motility

Abstract

detected by increased capacitance (capacitance should give a readout proportional to surface area, whether the membrane is folded or not)6,7. Lung epithelium may behave in a similar way to bladder epithelium8. Macrophages also increase their surface area as they engulf particles by phagocytosis: surface area increases as the cell extends a pseudopod around the particle, and then decreases when the phagosome is pinched off. Area increase can again be detected by capacitance and phagocytosis seems to depend on the exocytosis of membrane vesicles into the phagocytic cup9–11. The surface area of an irregular, motile cell is less easily measured. Using reconstructions from optical sections we found that Dictyostelium discoideum cells can increase their surface area by 30% over a few minutes5. We did not see an unfolding of the membrane that might contribute to this, and total internal reflection fluorescence microscopy did not reveal folding of the lower membrane12. It is difficult to exclude a contribution from sub-microscopic folds, but we prefer to propose that these cells regulate their surface area mostly by changing the balance between exocytosis and endocytosis. This would also explain why an operational endocytic cycle is essential for motility in D. discoideum cells13. Surface area regulation: underexplored yet crucial in cell motility

DOI: 10.1038/nrm2419-c2

Cite this paper

@article{Kay2008SurfaceAR, title={Surface area regulation: underexplored yet crucial in cell motility}, author={Robert R Kay and Paul D. Langridge and David Traynor and Oliver Hoeller}, journal={Nature Reviews Molecular Cell Biology}, year={2008}, volume={9}, pages={662-662} }