Suramin-treated HT29-D4 cells grown in the presence of glucose in permeable culture chambers form electrically active epithelial monolayers. A comparative study with HT29-D4 cells grown in the absence of glucose.

@article{Fantini1990SuramintreatedHC,
  title={Suramin-treated HT29-D4 cells grown in the presence of glucose in permeable culture chambers form electrically active epithelial monolayers. A comparative study with HT29-D4 cells grown in the absence of glucose.},
  author={Jacques Fantini and J. B. Rognoni and Bernard Verrier and Maxime Lehmann and Monique Roccabianca and Jean Mauchamp and Jacques Marvaldi},
  journal={European journal of cell biology},
  year={1990},
  volume={51 1},
  pages={110-9}
}
The clonal cell line HT29-D4 is able to differentiate by two different ways: i) by replacing glucose by galactose in the culture medium; ii) by addition of suramin (a drug known to interfere with the growth promoting activity of growth factors) in the medium. In both cases the transition in the organization of the cell monolayer occurred without cell loss… CONTINUE READING