Supramolecular Organization in Calf-Thymus DNA Solutions under Flow in Dependence with DNA Concentration

  title={Supramolecular Organization in Calf-Thymus DNA Solutions under Flow in Dependence with DNA Concentration},
  author={L. M. Bravo-Anaya and E. R. Mac{\'i}as and J. H. P{\'e}rez-L{\'o}pez and H. Galliard and D. Roux and G. Landazuri and F. C. Ramos and M. Rinaudo and F. Pignon and J. Soltero},
DNA dynamics and flow properties are of great importance for understanding its functions. DNA is a semiflexible polymer chain characterized by having a large persistence length of around 50 nm and high charge density; DNA chains are interacting efficiently at high concentrations, in dependence of the ionic concentration. In relation with DNA molecular characteristics, it is also known that DNA solutions are able to form liquid crystalline phases over a critical polymer concentration. In this… Expand
6 Citations
Role of Electrostatic Interactions on Supramolecular Organization in Calf-Thymus DNA Solutions under Flow
A critical transition is observed in rheological curves, where the viscosity decreases sharply at a given critical shear stress corresponding to a plateau in the σ(γ˙) plot also observed in creep transient experiment. Expand
Double-stranded and single-stranded well-entangled DNA solutions under LAOS: A comprehensive study
Abstract The nonlinear rheological behaviors of concentrated double-stranded (ds) and single-stranded (ss) DNA solutions were studied under standard oscillatory and large amplitude oscillatory shearExpand
Combining particle tracking microrheology and viscometry for the study of DNA aqueous solutions.
It is demonstrated that the combination of passive particle tracking with viscometry can provide a complete picture on the viscoelasticity of DNA-based biopolymer materials. Expand
Reconfigurable Liquids Stabilized by DNA Surfactants.
The design and construction of DNA microcapsules are described using the cooperative assembly of DNA and amine-functionalized polyhedral oligomeric silsesquioxane (POSS-NH2) at the oil-water interface, showing promising potentials for drug delivery, biphasic reactors, and programmable liquid constructs. Expand
The viscosity-radius relationship for concentrated polymer solutions
  • D. Dunstan
  • Materials Science, Physics
  • Scientific Reports
  • 2019
Scaling arguments and experimental evidence are used to determine the relationship between the viscosity, η, and chain radius of gyration, RG, and the thermal expansion coefficients determine the variation in the power law exponents that are measured for different polymer systems. Expand
Shear Induced Interactions Cause Polymer Compression
The analytical form of the force balance equations that incorporate the effect of shear induced particle pressure predict a reduced polymer blob size and reducing viscosity with increasing shear rate as has been observed experimentally. Expand


Conformation and Rheological Properties of Calf-Thymus DNA in Solution
Studies of DNA molecule behavior in aqueous solutions performed through different approaches allow assessment of the solute-solvent interactions and examination of the strong influence ofExpand
Multimolecular aggregation in concentrated isotropic solutions of mononucleosomal DNA in 1 M sodium chloride
Introduction. From a physical point of view, semiflexible polymer chains are interesting because the persistence segments have a large aspect ratio. For this reason, the interaction between twoExpand
Multimolecular aggregation of mononucleosomal DNA in concentrated isotropic solutions
We study semidilute and concentrated solutions of mononucleosomal DNA at three different NaCl concentrations by static and dynamic light scattering, viscosity, and electron cryomicroscopy. At lowExpand
Association of short DNA fragments: Steady state fluorescence polarization study
It is found that end‐over‐end tumbling rather than axial spinning and internal twisting motions are affected by aggregation/association, which implies that the aggregates observed are similar to those observed in a recently discovered DNA sol–gel transition. Expand
Preparation and Characterization of a Set of Linear DNA Molecules for Polymer Physics and Rheology Studies
Imaging of single DNA molecules has enabled detailed studies of dilute polymer dynamics and rigorous testing of assumptions and predictions of molecular theories. It is of interest to extend theseExpand
Control of the flow properties of DNA by topoisomerase II and its targeting inhibitor.
It is found that the rheology is critically dependent on the formation of temporal entanglements among the DNA molecules with a relaxation time of ∼1 s, and it is observed that topoisomerase II effectively removes these entanglement and transforms the solution from an elastic physical gel to a viscous fluid depending on the consumption of ATP. Expand
The Individualistic Dynamics of Entangled DNA in Solution
We present the direct visualizations of single, entangled DNA polymers in three flow experiments:  relaxation following a rapid shear deformation, steady shear, and startup shear. To evaluateExpand
Opticohydrodynamic properties of high‐molecular‐weight DNA. III. The effects of NaCl concentration
Excluded volume and persistence length of high‐molecular‐weight DNA from T2 bacteriophage have been evaluated over a range of NaCl concentrations from 0.005 to 2.0M using low‐shear flow‐birefringenceExpand
Shear Banding or Not in Entangled DNA Solutions
ntangled DNA solutions are ideal as a model system to examine nonlinear shear flow behavior. ven when the number of entanglements per chain, Z, is higher than 100, the solution is still soft noughExpand
The persistence length of DNA is reached from the persistence length of its null isomer through an internal electrostatic stretching force.
The accuracy for real polyelectrolytes of the Odijk-Skolnik-Fixman assumption that the polymer structure is invariant to changes in electrostatic forces is doubted, and the predicted dependence of P on salt concentration is log-c over most of the concentration range, with no tendency toward a salt-independent value in the range of validity of the theory. Expand