Supragingival plaque microbial analysis in reflection to caries experience


BACKGROUND Dental caries develops as a result of the metabolism of carbohydrates by cariogenic bacteria present in a complex biofilm. The present study aimed to examine if bacteria in pooled supragingival plaque samples quantified using a "checkerboard DNA-DNA hybridization" based panel of caries-related bacteria, could reflect the caries experience in a manner similar to saliva samples analysed using a chair-side method in a previous investigation. METHODS A total of 86 mothers and their children aged 4-6 years and 12-16 years old participated. Caries experience (DMFT/dmft; Decayed, Missing and Filled Teeth for permanent and primary teeth) was registered clinically and radiographically. Caries was recorded at the D3 level (caries into dentine). The D/d component was divided into three categories. A pooled supragingival plaque sample per participant was obtained from posterior approximal sites. Analyses of 15 bacterial species were performed using the checkerboard DNA-DNA hybridisation technique. RESULTS No significant relationships were found between the bacterial scores and DMFT/dmft nor D/d groups. CONCLUSIONS Unlike the saliva samples and the chair-side method, interproximal pooled plaque samples analysed using the "checkerboard DNA-DNA hybridization technique" did not reveal any significant relations between the bacterial counts and the caries experience.

DOI: 10.1186/1472-6831-13-5

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@inproceedings{Mannaa2013SupragingivalPM, title={Supragingival plaque microbial analysis in reflection to caries experience}, author={Alaa Mannaa and Anette Carl{\'e}n and Guglielmo Campus and Peter K Lingstr{\"{o}m}, booktitle={BMC oral health}, year={2013} }