Suppression of xylan endotransglycosylase PtxtXyn10A affects cellulose microfibril angle in secondary wall in aspen wood.

@article{DerbaMaceluch2015SuppressionOX,
  title={Suppression of xylan endotransglycosylase PtxtXyn10A affects cellulose microfibril angle in secondary wall in aspen wood.},
  author={Marta Derba-Maceluch and Tatsuya Awano and Junko Takahashi and Jessica Lucenius and Christine Ratke and Inkeri Kontro and Marta Busse-Wicher and Ondřej Kos{\'i}k and Ryo Tanaka and Anders Winz{\'e}ll and {\AA}sa M. Kallas and Joanna Leśniewska and Fredrik Berthold and Peter Immerzeel and Tuula T. Teeri and In{\'e}s Ezcurra and Paul Dupree and Ritva Serimaa and Ewa J Mellerowicz},
  journal={The New phytologist},
  year={2015},
  volume={205 2},
  pages={666-81}
}
Certain xylanases from family GH10 are highly expressed during secondary wall deposition, but their function is unknown. We carried out functional analyses of the secondary-wall specific PtxtXyn10A in hybrid aspen (Populus tremula × tremuloides). PtxtXyn10A function was analysed by expression studies, overexpression in Arabidopsis protoplasts and by downregulation in aspen. PtxtXyn10A overexpression in Arabidopsis protoplasts resulted in increased xylan endotransglycosylation rather than… CONTINUE READING
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