Suppression of transcription polarity in the Escherichia coli haemolysin operon by a short upstream element shared by polysaccharide and DNA transfer determinants.

@article{Nieto1996SuppressionOT,
  title={Suppression of transcription polarity in the Escherichia coli haemolysin operon by a short upstream element shared by polysaccharide and DNA transfer determinants.},
  author={Jos{\'e} Mar{\'i}a Nieto and M. J. Bailey and Colin Hughes and Vassilis Koronakis},
  journal={Molecular microbiology},
  year={1996},
  volume={19 4},
  pages={705-13}
}
Expression of the Escherichia coli hlyCABD operon encoding synthesis, maturation and export of haemolysin toxin was strongly dependent upon a 35 bp DNA sequence, spanning the element GGCGGTAG, located 2 kbp upstream. When the hly operon was placed under the control of the inducible tac promoter, expression remained dependent upon this element, when transcribed in its native orientation 3' of the promoter. The increase in ptac-directed transcription was strongest for the distal, export genes of… CONTINUE READING

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