Suppression of ribosomal reinitiation at upstream open reading frames in amino acid-starved cells forms the basis for GCN4 translational control.

@article{Abastado1991SuppressionOR,
  title={Suppression of ribosomal reinitiation at upstream open reading frames in amino acid-starved cells forms the basis for GCN4 translational control.},
  author={J. P. Abastado and Prof. Dr. F. Miller and Belinda M. Jackson and Alan G Hinnebusch},
  journal={Molecular and cellular biology},
  year={1991},
  volume={11 1},
  pages={486-96}
}
GCN4 encodes a transcriptional activator of amino acid-biosynthetic genes in Saccharomyces cerevisiae that is regulated at the translational level by upstream open reading frames (uORFs) in its mRNA leader. uORF4 (counting from the 5' end) is sufficient to repress GCN4 under nonstarvation conditions; uORF1 is required to overcome the inhibitory effect of uORF4 and stimulate GCN4 translation in amino acid-starved cells. Insertions of sequences with the potential to form secondary structure… CONTINUE READING