Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes.

@article{Buvoli2000SuppressionON,
  title={Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes.},
  author={Massimo Buvoli and Ada Buvoli and L. A. Leinwand},
  journal={Molecular and cellular biology},
  year={2000},
  volume={20 9},
  pages={3116-24}
}
We demonstrate here the first experimental suppression of a premature termination codon in vivo by using an ochre suppressor tRNA acting in an intact mouse. Multicopy tRNA expression plasmids were directly injected into skeletal muscle and into the hearts of transgenic mice carrying a reporter gene with an ochre mutation. A strategy for modulation of suppressor efficiency, applicable to diverse systems and based on tandem multimerization of the tRNA gene, is developed. The product of… CONTINUE READING

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