Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish.

@article{Kurita2003SuppressionOL,
  title={Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish.},
  author={Ryo Kurita and Hiroshi Sagara and Yutaka Aoki and Brian A. Link and Ken-ichi Arai and Sumiko Watanabe},
  journal={Developmental biology},
  year={2003},
  volume={255 1},
  pages={113-27}
}
In order to study lens-retina relationships during development, we cloned the zebrafish alphaA-crystallin cDNA and its promoter region. Using a 2.8-kb fragment of the zebrafish alphaA-crystallin promoter (z(alpha)Acry), we expressed the diphtheria toxin A fragment (DTA) in zebrafish embryos in a lens-specific manner. Injection of the z(alpha)Acry-DTA plasmid into eggs at the one-or two-cell stage resulted in the formation of small eyes, in which both lens and retina were reduced in size. In the… CONTINUE READING

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