Suppression of chlorophyll fluorescence during quantitative fluorescence microscopy of plant protoplasts

@article{McLellan1985SuppressionOC,
  title={Suppression of chlorophyll fluorescence during quantitative fluorescence microscopy of plant protoplasts},
  author={Mary S McLellan},
  journal={The Histochemical Journal},
  year={1985},
  volume={17},
  pages={1167-1168}
}
The excitation of chlorophyll molecules by wavelengths of 340-560 nm results in a red autofluorescent emission. This is useful in some studies of plant tissue or isolated protoplasts, e.g. protoplast fusion studies but can also interfere with quantitative studies of secondary fluorescence from specific probe molecules. This has proved to be a difficulty with cytometric measurements of protoplasts using the Leitz MPV compact flow cytometer and the MPV3 fluorescence microscope. This problem has… CONTINUE READING