Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice

@inproceedings{NishizawaYokoi2012SuppressionOK,
  title={Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice},
  author={Ayako Nishizawa-Yokoi and Satoko Nonaka and Hiroaki Saika and Yong-Ik Kwon and Keishi Osakabe and Seiichi Toki},
  booktitle={The New phytologist},
  year={2012}
}
Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in Agrobacterium-mediated transferred DNA (T-DNA) integration into the genome of the model plant Arabidopsis remains inconclusive. Having established a rapid and highly efficient Agrobacterium-mediated transformation system in rice (Oryza sativa) using scutellum-derived calli, we examined here the involvement of the NHEJ pathway in Agrobacterium-mediated stable transformation in rice. Rice calli from OsKu70, OsKu80 and… CONTINUE READING