Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane.

@article{Sousa2012SuppressionOE,
  title={Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane.},
  author={Leiliane P. Sousa and Irit Lax and Hongying Shen and Shawn M Ferguson and Pietro De Camilli and Joseph Schlessinger},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2012},
  volume={109 12},
  pages={4419-24}
}
The role of endocytosis in the control of EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts in which dynamin was conditionally depleted. Dynamin is a GTPase shown to play an important role in the control clathrin mediated endocytosis of EGFR and other cell surface receptors. In this report, we demonstrate that EGF binding activity and the display of high and low affinity EGFRs on the cell surface are not affected by dynamin depletion. By contrast, dynamin… CONTINUE READING