Suppression of Cdc2 Dephosphorylation at the Tyrosine 15 Residue during Nitrosourea-induced G2M phase Arrest in Glioblastoma Cell Lines

@article{Nakamizo2004SuppressionOC,
  title={Suppression of Cdc2 Dephosphorylation at the Tyrosine 15 Residue during Nitrosourea-induced G2M phase Arrest in Glioblastoma Cell Lines},
  author={A. Nakamizo and T. Inamura and S. Inoha and T. Amano and H. Ochi and K. Ikezaki and M. Fukui},
  journal={Journal of Neuro-Oncology},
  year={2004},
  volume={59},
  pages={7-13}
}
  • A. Nakamizo, T. Inamura, +4 authors M. Fukui
  • Published 2004
  • Medicine, Biology
  • Journal of Neuro-Oncology
  • We examined the mechanism of action of nitrosoureas as represented by 1-(4-amino-2-methyl-5-pyrimidinyl) methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU) with respect to p53 and the G2M cell cycle checkpoint using two glioblastoma cell lines: U251MG and U373MG, with mutated p53. At log-phase cell growth, fresh medium containing ACNU (final concentration, 3, 10, or 30 μg/ml) was added. After 24 h of incubation, cells were harvested for flow cytometric or Western analysis. In both lines, cell… CONTINUE READING
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