Suppressing the CRISPR/Cas adaptive immune system in bacterial infections

@article{Gholizadeh2017SuppressingTC,
  title={Suppressing the CRISPR/Cas adaptive immune system in bacterial infections},
  author={Pourya Gholizadeh and Mohammad Hossein Aghazadeh and Mohammad Asgharzadeh and Hossein Samadi Kafil},
  journal={European Journal of Clinical Microbiology \& Infectious Diseases},
  year={2017},
  volume={36},
  pages={2043-2051}
}
Clustered regularly interspaced short palindromic repeats (CRISPR) coupled with CRISPR-associated (Cas) proteins (CRISPR/Cas) are the adaptive immune system of eubacteria and archaebacteria. [] Key Result These results showed that the CRISPR/Cas system might prevent the emergence of virulence both in vitro and in vivo. Moreover, this system was shown to be a strong selective pressure for the acquisition of antibiotic resistance and virulence factor in bacterial pathogens.

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References

SHOWING 1-10 OF 88 REFERENCES

A CRISPR-CAS System Mediates Bacterial Innate Immune Evasion and Virulence

It is demonstrated that the Cas protein Cas9 of Francisella novicida uses a unique, small, CRISPR/Cas-associated RNA (scaRNA) to repress an endogenous transcript encoding a bacterial lipoprotein.

Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system

The first examples of genes that mediate the inhibition of a CRISPR/Cas system are described, found in the genomes of bacteriophages infecting Pseudomonas aeruginosa and provides new insight into the co-evolution of phages and bacteria.

CRISPR/Cas, the Immune System of Bacteria and Archaea

Clustered regularly interspaced short palindromic repeats (CRISPR) form peculiar genetic loci, which provide acquired immunity against viruses and plasmids by targeting nucleic acid in a sequence-specific manner.

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

In vivo evidence is provided that the Streptococcus thermophilus CRISPR1/Cas system can also naturally acquire spacers from a self-replicating plasmid containing an antibiotic-resistance gene, leading toplasmid loss.

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

The results show that active CRISPR/Cas systems can be transferred across distant genera and provide heterologous interference against invasive nucleic acids and can be leveraged to develop strains more robust against phage attack, and safer organisms less likely to uptake and disseminate plasmid-encoded undesirable genetic elements.

CRISPR-based adaptive and heritable immunity in prokaryotes.

The CRISPR/Cas Adaptive Immune System of Pseudomonas aeruginosa Mediates Resistance to Naturally Occurring and Engineered Phages

These phages are only the second identified group of naturally occurring phages demonstrated to be blocked for replication by a nonengineered CRISPR/Cas system, and the results provide the first evidence that the P. aeruginosa type I-F CRIS PR-Cas system can function in phage resistance.

Multiple mechanisms for CRISPR–Cas inhibition by anti-CRISPR proteins

The first examples of proteins produced by phages that inhibit a CRISPR–Cas system are identified, and the diverse sequences and mechanisms of action of these anti-CRISPR proteins imply an independent evolution, and foreshadow the existence of other means by which proteins may alter CRISpr–Cas function.
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