Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos.

@article{Botigelli2018SupplementingIV,
  title={Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos.},
  author={Ramon Cesar Botigelli and Eduardo Montanari Razza and Elisa Mariano Pioltine and Patr{\'i}cia Kubo Fontes and K{\'a}tia R L Schwarz and Cl{\'a}udia Lima Verde Leal and Marcelo F{\'a}bio Gouveia Nogueira},
  journal={Reproductive biology},
  year={2018},
  volume={18 1},
  pages={66-75}
}
In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. We evaluated the effects on the lipid content (LC) of oocytes and embryos and also verified the expression of 96 transcripts related to… CONTINUE READING