Supplemental Data S 1 Ubiquitination of RIP 1 Regulates an NFk B-Independent Cell-Death Switch in TNF Signaling

Cell Lines SVT35 is a derivative of the Jurkat T cell line that is stably transfected with a cell-surface CD14 reporter gene downstream of eight NF-kBbinding sites [S1]. The RIP1-deficient cell line 35.3.13 was generated from the parental SVT35 cell line by ICR 191 mutagenesis and selection of TNF-unresponsive but PMA/ionomycin-responsive mutant cells [S1… CONTINUE READING